Journal: Journal of Ginseng Research

Article Title: The ectopic olfactory receptor OR7A17 regulates the proliferation and differentiation of human epidermal keratinocytes, and ginsenoside Rh3 acts as its antagonist

doi: 10.1016/j.jgr.2025.100972

Figure Lengend Snippet: OR7A17 overexpression increases proliferation of HaCaT cells. (A) OR7A17 expression levels in HaCaT cells were measured by RT-qPCR and (B) Western blot analysis. (C) The effects of OR7A17 on proliferation were determined by BrdU ELISA and (D) EdU incorporation assays. (E) Flow cytometry analysis by PI staining. Mock-HaCaT cells were incubated with 10 nM TPA for 90 min. Representative histogram of the gated cells in the G1, S, and G2 phases. (F) The graph illustrates the percentage of cells in each phase (G) RT-qPCR was performed to determine mRNA levels of cyclins D1 and E. (H) Western blotting was performed to determine the expression levels of cyclin D1, cyclin E, p21, and p-Rb. (I) A clonogenic assay was performed on OR7A17-HaCaT cells. The images were taken following a two-week incubation of the cells (∗p < 0.05 vs. Mock, ∗∗p < 0.01 vs. Mock).

Article Snippet: Antibodies against JNK, p-JNK, p-ERK 1/2, ERK 1/2, p-p38 MAPK, p38 MAPK, p-NF-κB, NF-κB, p-cyclic AMP-response element-binding protein (CREB), CREB, Cyclin D1, and Cyclin E were sourced from Santa Cruz Biotechnology.

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Staining, Incubation, Clonogenic Assay